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Rhodamine B 500%

C.I. Basic Violet 10 - 45170

CodePack SizePrice (Inc. VAT + Postage)
125X 10g £5.72Add to Cart
125H 25g £8.32Add to Cart
125I 50g £12.68Add to Cart
125V 100g £19.50Add to Cart
125S 500g £65.00Add to Cart
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RHODAMINE B - 500%

IUPAC Name: [9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride


Formula: C28H31ClN2O3

CAS No: 81-88-9

Mol Wt: 479.02

Solubility in water: ~50 g/l

Rhodamine B is often used as a tracer dye within water to determine the rate and direction of flow. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid fast organisms, notably mycrobaerium.

Rhodamine B is tunable around 610 nm when used as a laser dye. Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol 1.0 and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent.

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